Separation and characterization of urinary indoles resembling 5-hydroxytryptamine and tryptamine

Abstract

METHODS Materials.'AnalaR'grade solvents and water were redistilled in an all-glass still. Sodium phosphate buffer (0.5M), pH 6, was prepared fromNa2HPO4, 2H20, withconc. HCI to adjust the pH (glass electrode). Acetic acid (0-02N) in 75%(v/v) ethanol (eluting agent) …